osteogenic media (om)  (Lonza)

Journal: International Journal of Spine Surgery

Article Title: Cell and Tissue Response to Polyethylene Terephthalate Mesh Containing Bone Allograft in Vitro and in Vivo

doi: 10.14444/7135

Figure Lengend Snippet: Design of human mesenchymal stromal cell experiment.

Article Snippet: In Vitro Culture Human MSCs (male donor) and normal human osteoblasts (NHOst cells, male donor), as well as MSC growth media (MSCGM) and osteogenic media (OM), were purchased from Lonza (Basel, Switzerland).

Techniques:

Journal: International Journal of Spine Surgery

Article Title: Cell and Tissue Response to Polyethylene Terephthalate Mesh Containing Bone Allograft in Vitro and in Vivo

doi: 10.14444/7135

Figure Lengend Snippet: In vitro bone marrow stromal cell (MSC) response of cells cultured on tissue culture plastic (TCPS) or mineralized bone matrix (MBM), with MSC growth media (GM) or osteogenic media (OM), in the presence or absence of polyethylene terephthalate mesh (PET). Response was examined by measuring markers that indicate the initiation of differentiation including (A) DNA; (B) alkaline phosphate specific activity; factors that indicate late stage osteoblast differentiation including (C) osteocalcin and (D) osteoprotegerin (OPG); and production of factors associated with osteogenesis, including bone morphogenetic proteins (BMPs) 2 (E) and 4 (F) and (G) vascular endothelial growth factor-A (VEGF). P < .05 # versus no mesh, @ versus no MBM. % versus GM.

Article Snippet: In Vitro Culture Human MSCs (male donor) and normal human osteoblasts (NHOst cells, male donor), as well as MSC growth media (MSCGM) and osteogenic media (OM), were purchased from Lonza (Basel, Switzerland).

Techniques: In Vitro, Cell Culture, Activity Assay

Journal: Journal of Biomedical Materials Research. Part a

Article Title: The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

doi: 10.1002/jbm.a.36223

Figure Lengend Snippet: SaOS‐2 cell viability and cell number subject to different PVPA‐co‐AA polymer treatments at different concentrations (A) Representative SaOS‐2 cells subject to a range of PVPA‐co‐AA polymer treatments at different concentrations stained with fluorescent Live/Dead Double Staining at day 7 day; live cells appear green, dead cells appear red. (B) SaOS‐2 cell number subject to PVPA‐co‐AA polymer treatments at different concentrations measured by AlamarBlue® Cell Viability assay at day 0, 1, 3, 7. The graph shows means ± SD of data. Asterisks indicate significant (* p < 0.05, ** p < 0.01, *** p < 0.001) difference in fold change vs. the control at the same time point.

Article Snippet: After 72 h media was replaced with the SaOS‐2 osteogenic media (SaOS‐2 OM), which was the DMEM supplemented with 0.5% FCS, antibiotics (100 U/mL Penicillin, 100 mg/mL streptomycin), 10 nM dexamethasone and 50 μg/mL L‐ascorbic acid (Sigma‐Aldrich, UK).

Techniques: Staining, Double Staining, Viability Assay

Journal: Journal of Biomedical Materials Research. Part a

Article Title: The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

doi: 10.1002/jbm.a.36223

Figure Lengend Snippet: (A) Calcium chelation capacity of the different PVPA‐co‐AA polymer at pH 7.3 and (B) the maximum value of SaOS‐2 cells mineralization with the different PVPA‐co‐AA polymer treatments.

Article Snippet: After 72 h media was replaced with the SaOS‐2 osteogenic media (SaOS‐2 OM), which was the DMEM supplemented with 0.5% FCS, antibiotics (100 U/mL Penicillin, 100 mg/mL streptomycin), 10 nM dexamethasone and 50 μg/mL L‐ascorbic acid (Sigma‐Aldrich, UK).

Techniques:

Journal: Journal of Biomedical Materials Research. Part a

Article Title: The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

doi: 10.1002/jbm.a.36223

Figure Lengend Snippet: Effect of different PVPA‐co‐AA polymer treatments at different concentrations on SaOS‐2 cells mineralization. (A) Representative photos showing the patterns of alizarin red S staining of the in vitro mineralization matrix subject to PVPA‐co‐AA polymer treatments at different concentrations. (B) Shows the quantified percentage of SaOS‐2 cells mineralization subject to PVPA‐co‐AA polymer treatments at different concentrations. The graph shows means ± SD of data. ± indicates the significant increase or decrease (at least p < 0.05) in the mineralization percentage vs. the control samples.

Article Snippet: After 72 h media was replaced with the SaOS‐2 osteogenic media (SaOS‐2 OM), which was the DMEM supplemented with 0.5% FCS, antibiotics (100 U/mL Penicillin, 100 mg/mL streptomycin), 10 nM dexamethasone and 50 μg/mL L‐ascorbic acid (Sigma‐Aldrich, UK).

Techniques: Staining, In Vitro

Journal: Journal of Biomedical Materials Research. Part a

Article Title: The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

doi: 10.1002/jbm.a.36223

Figure Lengend Snippet: Representative SEM images (A and B) and a comparison with Alizarin red mineralization staining (C). SEM images demonstrate the differences in the polymer‐calcium complexes, indicative of the chelation capacities of the different polymer ratios, whereby the increasing amount of VPA (0, 34, and 100 mol % VPA) show increased levels of granulation/particle formation when exposed to the same concentrations of Ca2+ ions (A). The subsequent effects of the different AA:VPA ratio on the culture of SaOS‐2 cells, is demonstrated with a lower magnification image is inset (B); the differences in particle formation of morphology of particles was considered to play a role in the mineralization behavior in association with cells. Dark areas are indicative of acellular regions on the surface of the thermanox coverslip and appear featureless in contrast with the bright areas of mineralized material. In comparison to the SEM the calcium deposition observed by SEM could be correlated to the mineralization observed by alizarin red staining for calcium (C), where increasing the mol % VPA resulted in more agglomerated clusters of mineralization, whereas P‐34 demonstrated a much more homogenous layer of mineralization with the presence of nodulelike formations.

Article Snippet: After 72 h media was replaced with the SaOS‐2 osteogenic media (SaOS‐2 OM), which was the DMEM supplemented with 0.5% FCS, antibiotics (100 U/mL Penicillin, 100 mg/mL streptomycin), 10 nM dexamethasone and 50 μg/mL L‐ascorbic acid (Sigma‐Aldrich, UK).

Techniques: Staining

Journal: Journal of Biomedical Materials Research. Part a

Article Title: The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

doi: 10.1002/jbm.a.36223

Figure Lengend Snippet: Osteogenic effect of PVPA‐co‐AA polymer in SaOS‐2 cells and human BM‐MSCs. Representative photos showing the patterns and quantified percentage of ALP, in‐vitro mineralization, and collagen staining of (A) human BM‐MSCs and (B) SaOS‐2 cells subject to P‐34 polymer treatments at different concentrations. The graph shows means ± SD of data. Asterisks indicate significant (* p < 0.05, ** p < 0.01, *** p < 0.001) increase in the staining percentage vs. the control at the same time point.

Article Snippet: After 72 h media was replaced with the SaOS‐2 osteogenic media (SaOS‐2 OM), which was the DMEM supplemented with 0.5% FCS, antibiotics (100 U/mL Penicillin, 100 mg/mL streptomycin), 10 nM dexamethasone and 50 μg/mL L‐ascorbic acid (Sigma‐Aldrich, UK).

Techniques: In Vitro, Staining

Journal: Journal of Biomedical Materials Research. Part a

Article Title: The unique calcium chelation property of poly(vinyl phosphonic acid‐co‐acrylic acid) and effects on osteogenesis in vitro

doi: 10.1002/jbm.a.36223

Figure Lengend Snippet: Osteogenic marker gene expression in SaOS‐2 cells and human BM‐MSCs. (A) shows the osteogenic marker gene expression in human BM‐MSCs at day 21 and 28, subject to P‐34 polymer treatments at different concentrations. (B) shows the osteogenic marker gene expression in SaOS‐2 cells at day 1 and 7, subject to P‐34 polymer treatments at different concentrations. The data were normalized to housekeeping gene GAPDH rRNA and represent mean ± SD. Asterisks indicate significant (* p < 0.05, ** p < 0.01, *** p < 0.001) difference between the samples at the same time point.

Article Snippet: After 72 h media was replaced with the SaOS‐2 osteogenic media (SaOS‐2 OM), which was the DMEM supplemented with 0.5% FCS, antibiotics (100 U/mL Penicillin, 100 mg/mL streptomycin), 10 nM dexamethasone and 50 μg/mL L‐ascorbic acid (Sigma‐Aldrich, UK).

Techniques: Marker, Expressing